Letter Extended Data Figure 5-e,fのGFPとBFPの共挿入ES細胞の入ったGOFのFI-SCではないのですか。これは若山研に無いESですから丹羽研の提供だとすると、CD1のTSも丹羽研提供ですから、このESもCD1であった可能性はありますよね。無論GOFのFI-SCはあったに決まっている。だって、小保方さんは黒いマウスを渡されているんでしょ。彼女は混乱の中で若山さんをかばうために何を渡されたか知らなかったとまで答えていますね。なんてかわいそうなんだろう。以上です。
However, as Fgf4-induced stem cells lay between STAP stem cells and trophoblast stem cells in the dendrogram, the possibility of contamination of STAP stem cells in the Fgf4-induced stem-cell population cannot be ruled out. Previous studies have indicated that inner cell mass (ICM)-type pluripotent cells can be removed from culture by treating the culture with a JAK inhibitor16 (Extended Data Fig. 5a, b). In contrast, the JAK inhibitor treatment had no substantial effect on Oct4-GFP expression in Fgf4-induced stem-cell culture (Extended Data Fig. 5c, d; see Extended Data Fig. 5e, f for control). Expression of neither pluripotency markers (Fig. 2j) nor trophoblast markers (Fig. 2k) was substantially affected, indicating that pluripotency marker expression is unlikely to reflect contaminating STAP stem cells (ICM-type). Consistent with this idea, Fgf4-induced stem cells that were strongly positive for the trophoblast marker Itga7 (a surface marker for trophoblasts but not ES cells) also expressed high levels of Oct4-GFP (Extended Data Fig. 5g).
a-f, JAK inhibitor treatment assay for Fgf4-induced stem cells. Fgf4-induced stem cells were cultured under feeder-free conditions and treated with 0.6M JAK inhibitor for 48h. JAK inhibitor treatment assay eliminated ES cells (Oct4-GFP+) from the culture (a, b). The level of Oct4-GFP expression in Fgf4-induced stem cells, which was moderate, was maintained even after JAK inhibitor treatment (c, d; three independent experiments). e, f, For an additional control, Fgf4-induced stem cells were plated in trophoblast stem-cell medium containing Fgf4 together with Oct4-GFP ES cells that constitutively expressed BFP (the number of plated cells was one-tenth of that of plated Fgf4-induced stem cells). Whereas BFP-expressing colonies (ES-cell-derived) still expressed Oct4-GFP in trophoblast stem-cell culture medium after 2 days (e), no Oct4-GFP+ colonies from BFP-expressing ES cells were observed in the JAK-inhibitor-treated culture (f).
g, FACS analysis of integrin 7 expression in Fgf4-induced stem cells. Over 40% of Fgf4-induced stem cells strongly expressed both the pluripotency marker Oct4-GFP and the trophoblast marker integrin 7. The bottom panel shows an isotype control for integrin 7 antibody. In ES cells, integrin-7-expressing cells were less than 0.1% (data not shown; three independent ES cell lines were examined).